Automated efficiency with manual control
QPatch® Compact is a flexible semi-automated patch clamp solution, allowing complete control of gigaseal ion channel recordings in up to 8 cells in parallel. The bench-top system offers an effortless and accurate patch clamp experience with minimum set-up and maintenance required. It is ideal for busy laboratories that require higher throughput than manual patch clamping can provide or laboratories that need electrophysiology data but do not have trained patch clampers.
With up to 8 sites in parallel, QPatch Compact provides sufficient throughput to rapidly obtain replicas and fuels data generation for your next publication – or medicinal chemistry program. In addition, it has proven to be a valuable tool for graduate and postgraduate teaching.
QPatch Compact offers:
- Giga-Ω seal in physiological Ringer’s solutions – no need for seal enhancers or fluoride
- Water-based temperature control at each measurement site
- Recording in up to 8 cells asynchronously in parallel
- Sophion’s unique adaptive current clamp feature
- Manual liquid additions supported by light and audio guidance
- On the run changing of protocols
- Sophion Analyzer is designed for easy and automated data analysis
Also, the unique design of the QPlates ensures consistent quality recordings time after time:
- Fast and complete liquid exchange via microfluidic channels
- Glass surfaces to prevent adherence of sticky compounds
- Ready-to-use individual electrode pairs – no need to ever re-chloride electrodes again
Discover how QPatch Compact can help you?
QPatch Compact is designed for busy laboratories that need quick access to patch-clamp data and lack the time or patience to master the art of operating a manual patch-clamp rig. With QPatch Compact, anyone can learn to patch-clamp in less than half an hour.
Take full advantage of QPatch Compact depending on your daily working requirements:
Automated electrophysiology with manual pipetting
The guided workflow for QPatch Compact makes it easy to learn and understand. With QPatch Compact, we claim that any lab-trained staff can learn how to patch clamp in less than ½ an hour.
The ease of use combined with the manual handling also makes the instrument well suited for educational purposes.
The colour blue denotes steps with physical interaction, inserting or adding plates or liquids to the rig.Throughput, metadata, compounds and protocols are added and selected on the responsive user interface, which also continuously provides information about cell status and experimental results so that e-phys protocols can be adapted and changed on the go.
Key QPatch Compact functionalities
Adaptive whole cell protocols
All cells are different, and they should be treated accordingly. QPatch Compact includes individual whole-cell protocols to increase whole-cell rates and ultimately the success rate for your experiment. Read more
Adaptive current clamp
The main challenge with current clamp on automated patch clamp platforms is the variation in sealing and resting membrane potential from cell to cell. This makes it impossible to design one current clamp protocol to work on all cells. We have solved that with our unique Adaptive Current Clamp feature. Read more
Adaptive voltage clamp
Optimize voltage clamp protocols individually for each cell’s electrical properties. The QPatch Compact system will automatically perform Boltzmann fits for each cell online. This is to estimate the cell-specific voltage needed to activate X% of the receptor population or the voltage that keeps X% of the cells in an inactivated state. Read more
Temperature control
With the temperature control add-on, you can perform experiments at controlled physiological temperatures. Thereby, reducing fluctuations caused by temperature changes in your laboratory environment and thus ensuring higher repeatability and reproducibility. Read more
Rs compensation
Series resistance compensation for fast-activating ion channels can help remove voltage clamp errors and improve clamp accuracy. All Sophion’s patch-clamp amplifiers utilize a patented technology enabling up to 100% Rs compensation. Read more
Sophion Analyzer software
Advanced and customized analysis of your data can be performed immediately after your data has been collected. Sophion Analyzer can analyze thousands of data points automatically and present the results according to your requirements. Read more
QPlate microflow based glass consumables using single & multi-hole technology
QPlates are based on microfluidic principles to ensure fast and complete liquid exchange, pre-defined compound concentration, and low compound consumption. Our QPlates come with one patch hole per measurement site or 10 patch holes per measurement site. Read more
